Synthesis of gold nanoparticles from Metarhizium anisopliae for management of blast disease of rice and its effect on soil biological index and physicochemical properties
Year: 2022
Type: preprint
Abstract: Aims: The aim of this experiment was to synthesized gold nanoparticles from entomopathogen fungi Metarhizium anisopliae, an indigenous biocntrol agent and its antifungal activity against Magnaporthe grisea and its effect on soil biological index and physicochemical properties. Methods:Biosynthesis of gold nanoparticles was done by following standard protocol and characterized by different equipments. soil biological and ph ysicochemical properties was carried out by following standard protocol. Results:Formation of gold nanoparticles were confirmed by UV-VIS spectroscopy study with absorption peaks at 550 nm. FTIR study showed that synthesized gold nanoparticle has all the required functional groups like OH, N-H, C-H and COO-. Study on surface properties of nanoparticles by using zetasizer resulted that gold nanoparticle from Metarhizium anisopliae was found to be negative and were stable in nature with zeta potential value of -20.7 mV. DLS analysis showed that the average size of the biosynthesized gold nanoparticles is 32.54 nm with polydispersity index of 0.560. TEM study showed that shape of the biosynthesized nanoparticle is from triangular to quasihedral and the size range from 9-54nm. Antifungal activity of gold nanoparticles at 150 ppm significantly inhibit the mycelia growth of the pathogens as compared to the Tryclozole @ 600 ppm Conclusions : A positive effect was found on soil biological index and physicochemical properties of soil along with reduction of disease incidence when rice seedling was treated as seedling dip treatment + foliar spray + Soil application @ 150ppm of biosynthesized gold nanoparticles.
Source: Research Square
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
SDG: Zero hunger
Open Access status: green
OpenAlex ID: W4297983233