Work

Protocol for intranasal chemoprevention delivery in a urethane mouse lung cancer model

Year: 2022

Type: article

Abstract: Mouse iloprost lung cancer chemoprevention studies typically use oral delivery. Here, we present a protocol for intranasal iloprost delivery within a urethane lung adenocarcinoma mouse model. We detail steps for intraperitoneal urethane injection in mice, followed by nine-week monitoring, intranasal iloprost treatment, and lungs harvesting for analysis. This iloprost delivery approach parallels an ongoing phase II clinical trial of inhaled iloprost for lung cancer chemoprevention. This protocol diversifies options for chemoprevention studies and offers a relevant and translatable model. For complete details on the use and execution of this protocol, please refer to Sompel et al. (2022).

Source: STAR Protocols

DOI: 10.1016/j.xpro.2022.101750

Authors: Lori Dwyer-Nield, Robert L. Keith, Meredith A. Tennis

Institutions: United States Department of Veterans Affairs, University of Colorado Anschutz

Language: English

Topic: Inhalation and Respiratory Drug Delivery

Subfield: Pulmonary and Respiratory Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,500