Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis
Year: 2022
Type: article
Abstract: Sexual reproduction in angiosperms requires the production and delivery of two male gametes by a three-celled haploid male gametophyte. This demands synchronized gene expression in a short developmental window to ensure double fertilization and seed set. While transcriptomic changes in developing pollen are known for Arabidopsis, no studies have integrated RNA and proteomic data in this model. Further, the role of alternative splicing has not been fully addressed, yet post-transcriptional and post-translational regulation may have a key role in gene expression dynamics during microgametogenesis. We have refined and substantially updated global transcriptomic and proteomic changes in developing pollen for two Arabidopsis accessions. Despite the superiority of RNA-seq over microarray-based platforms, we demonstrate high reproducibility and comparability. We identify thousands of long non-coding RNAs as potential regulators of pollen development, hundreds of changes in alternative splicing and provide insight into mRNA translation rate and storage in developing pollen. Our analysis delivers an integrated perspective of gene expression dynamics in developing Arabidopsis pollen and a foundation for studying the role of alternative splicing in this model.
Source: Plant Reproduction
Institutions: Czech Academy of Sciences, Charles University, Czech Academy of Sciences, Institute of Experimental Botany, Central European Institute of Technology – Masaryk University, Instituto Gulbenkian de Ciência, University of Leicester, KWS Saat (Germany), Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Masaryk University
Language: English
Topic: Plant Reproductive Biology
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,860
Awards: The role of Arabidopsis Lorelei-like GPI anchored proteins (LLGs) in pollen tube reception by the female gametophyte, G1445951237, The application of CRISPR-Cas9 for the creation of multiple mutants in the genes coding for the nascent polypeptide associated complex, Revealing the role of the nascent polypeptide associated complex during flower and fruit development of Arabidopsis thaliana, G5133807595, e-Infrastructure CZ, 14 ERA-CAPS. EVOREPRO. Evolution of Sexual Reproduction in Plants, Characterization of nuclear proteomes in the male germline and their implications under standard and stress conditions, Translation regulation in plant dormant reproduction units - pollen and seeds, Evolução da reprodução sexual em plantas, Nuclear regulatory landscape of bZIP transcription factors in plant reproductive development (REPROZIP), G770842330, Czech Infrastructure for Integrative Structural Biology
OpenAlex ID: W4307201117