Overexpression of transcriptional regulator and tailoring enzyme leads to the discovery of anti-inflammatory meroterpenoids from marine-derived fungus Alternaria alternata JJY-32
Year: 2022
Type: article
Abstract: Four new ACTG-toxins like meroterpenoids tricycloalternarenes O-R were discovered from a marine-derived fungus Alternaria alternata JJY-32 by the overexpression of a LaeA-like global transcriptional regulator and a MpaB-like biosynthetic tailoring enzyme. Among those structures, tricycloalternarene O possesses a non-canonical cyclohexanone-pyran fused furan ring structure and tricycloalternarene R contains a truncated isoprenoid terminal. The structures were elucidated based on comprehensive 1D and 2D NMR data and assisted by ECD calculations. Tricycloalternarenes O-R showed potent anti-inflammatory activity in TLR4-transfected microphage cells (RAW264.7).
Source: Frontiers in Marine Science
Authors: Guowei Liu, Dan Liu, Zongyuan Li, Jieying Jiao, Xuewen Hou, Xiaomin Zhang, Qian Che, Tianjiao Zhu, Dehai Li, Guojian Zhang
Language: English
Subfield: Applied Microbiology and Biotechnology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,950
Funders: Taishan Scholar Foundation of Shandong Province, National Natural Science Foundation of China, Natural Science Foundation of Shandong Province, Polit National Laboratory for Marine Science and Technology, Science and Technology Development Plan of Shandong Province, National Natural Science Foundation of China-Shandong Joint Fund, Fundamental Research Funds for the Central Universities
Awards: G1387120401, G158013351, G1623742816, G1743940349, G2184318899, G2236236896, G2296084301, G2555560632, G3945544845, G3983370572, G4715275366, G4815177905, G5031234603, G6177706599, G6492539748, G6653481752, G6669072966, G6985915429, G7008525000, G7027777623, G7267264738, G8177304663, G8505436922, G8786148109, G95675335
OpenAlex ID: W4307706232