Work

A Minimal Replicon Enables Efficacious, Species-Specific Gene Deletion in Chlamydia and Extension of Gene Knockout Studies to the Animal Model of Infection Using Chlamydia muridarum

Year: 2022

Type: article

Abstract: invasion and fitness assays. The virulence of the C. muridarum strains was then assessed in a murine infection model. Our approach represents a novel and efficient strategy for targeted genetic manipulation in Chlamydia beyond C. trachomatis L2. This advance will support comparative studies of species-specific infection biology and enable studies in a well-established murine model of chlamydial pathogenesis.

Source: Infection and Immunity

DOI: 10.1128/iai.00453-22

Authors: Kenneth A. Fields, Maria D. Bodero, Kaylyn R. Scanlon, Travis J. Jewett, Katerina Wolf

Institutions: University of Kentucky, University of Central Florida

Language: English

Topic: Reproductive tract infections research

Subfield: Microbiology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Institute of Allergy and Infectious Diseases