Aeromoniasis Induced Biochemical and Histopathological Alterations in the Head Kidney of Labeorohita
Year: 2022
Type: article
Abstract: ABSTRACT This study evaluated the effect of multiple doses of Aeromonasliquifaciens on the protein and DNA content and histopathalogical changes of head kidney of Labeorohita. Two experimental (Group-A, 10-2 + 10-2 CFU/fish; Group B, 10-3 + 10-3 CFU/fish) and two control (Groups a and b) groups of fish (66 in each group) were maintained for experimental purpose. Experimental groups (A and B) of fish received A. liquifaciens intramuscularly Six fish from experimental and control groups were sacrificed at hour 1, 3, 6, 12, 18, 24, 36, 48, 72, 96 and 216 of experimental period. The level of protein and DNA was found to be decreased throughout the infection period in both the experimental groups (A and B) when compared with their counterpart control groups (a and b). Head kidney showed inflammatory reaction, necrosis and glomeruli enlargement and congestion in the infected groups. Key words: Aeromoniasis, protein, DNA, Head Kidney, Ladeorohita REFERENCES Ali, K.S., Dorgai, L., Gazdag, A., Abraham, M. and Hermesz, E. (2003). Identification and induction of hsp 70 ene by heat shock and cadmium exposure in carp. Acta. Biol. Hung, 54(3-4): 323-334. Ankamma, N and VivekaVardhani, V(2017).Effect of dietary Aqua fix on qualitative protein profile in skeletal muscles of Labeo rohita.Biolife.5(2), pp279-283.doi:10.17812/blj.2017.5219 Ashley, P.J. (2007) Fish welfare: Current Issues in aquaculture. Appl.Anim.Behav.Sci.104 : 199-235 Azad, I.S., Rajendran, K.V., Raja, J.J.S., Vijayan, K.K. and Santiago, T.C.(2001) Virulence and histopathology of Aeromonashydrophila strains to tissue cultures. Microbios.77: 47-55 Begum, G. (2004). Carbofuran insecticide induced biochemical alterations in liver and muscle tissues of the fish Clariasbatrachus(Linn.) and recovery response. Aquatic Toxicol., 66(1): 83-92. Boone, A.N., and Vijayan, M.M. (2002). Constitutive heat shock protein 70 (HSC 70) expression in rainbow trout hepataytes: effect of heat shock and heavy metal exposure. Comp. Biochem. Physiol. Toxicol. Pharmacol., 132(2): 223-233. Coz-Rakovac R., Strunjak-Perovic I., Hacmanjek M., Topic PN., Lipez Z, and Sostaric B. (2005). Blood chemistry and histological properties of wild and cultured sea bass (Dicentrachuslabrax) in the North Adriatic Sea. Vet Res Commun, 29: 677-687. Dharansingh. KamleshNath., Trivedi S.P. and Sharma Y.K. (2008) Impact of copper on haematological profile of freshwaterfishChanna punctatus, J. Environ. Biol. 29(2):253-257. Gupta, S.K., Pal, A.K., Sahu, N.P., Dalvi, R., Kumar, V. and Mukherjee, S.C. (2008). Microbial levan in the diet of LabeorohitaHamilton juveniles: effect on non-specific immunity and histopathological changes after challenge with Aeromonashydrophila. J. Fish Dis. 31: 649-657. Swapna Gurrapu and Estari Mamidala. In vitro HIV-Reverse Transcriptase Inhibition of Andrographolide Isolatedfrom Andrographis Paniculata. European Journal of Biomedical and Pharmaceutical Sciences. 2017. Volume 4, Issue 12. 516-522. Karunasagar, I., Rosalind, G. and Karunasagar, I. (1991). Immunological responses of the Indian major carps to Aeromonashydrophilavaccine. J. Fish. Dis. 14: 413-417. Lamers, C.H.J. and Van Muiswinkel, W.B. (1986). Natural and acquired agglutinin to Aeromonashydrophilain carp. Cyprinuscarpio. Can. J. Fish Aquat. Sci. 43: 619-624. Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951). Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265-275. Mohanty, B.R., Mishra, J., Das, S., Jena, J.K. and Sahoo, P.K. (2008). An outbreak of aeromoniasis in an organised composite carp culture farm in India: experimental pathogenicity and antibiogram study. J. Aquaculture, 16: 27-37. Newman, S.G. (1993). Bacterial vaccines for fish. Ann. Rev. Fish Dis. 3: 145-185. Tabche, M.L., Gomes, O.L., Galar, M.M. and Lopez, L.E. (2002). Stress proteins produced by contaminated sediments with nickel in a pond with rainbow trout Oncorhynchus mykiss (Pisces: Salmonide). Rev. Biol. Trop., 50(3-4): 1159-1168. Welch, W.J. (1993). How cells respond to stress. Sci. Am. 268: 56-64. Yardimci Y.L. and Aydin N.L., (2011). Pathological findings of experimental Aeromonashydrophilainfection in Nile tilapia (Oreochromisniloticus). Ankara Univ. Vet. Fak. Derg. 58: 47-54.
Authors: Satyalatha B.D.J, VivekaVardhani.V
Institution: Acharya Nagarjuna University
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4310768898