Work

High-throughput sorting of two-color fluorescent-labeled zebrafish embryos

Year: 2022

Type: article

Abstract: The zebrafish embryos were widely employed in genetics, development and drug discovery studies as miniatured animal models. Sorting of two-color fluorescent embryos is often required in large-scale experiments but it is challenging to manually sort with high efficiency. Here, we reported a high-throughput sorting system for two-color fluorescent zebrafish embryos. The embryos can be automatically loaded from a sample pool and sorted based on the average fluorescent intensity. The two-color fluorescent signals were split into two lines and detected by an area array camera. The system achieves the sorting of 100 embryos in less than 10[Formula: see text]min with an accuracy of greater than 95%.

Source: Journal of Innovative Optical Health Sciences

DOI: 10.1142/s1793545823500037

Authors: Hongzhen Tang, Linbo Wang, Xiaohu Chen, Chong Chen, Hui Li, Guang Yang

Institutions: Changchun University of Science and Technology, Chinese Academy of Sciences, Suzhou Institute of Biomedical Engineering and Technology

Language: English

Topic: Zebrafish Biomedical Research Applications

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,500

Funders: National Natural Science Foundation of China, Jiangsu Provincial Key Research and Development Program