Methyl Jasmonate Modulates Feeding Behaviors and Hypothalamic Expression of the Orexin 1 Receptor in Rats
Year: 2023
Type: article
Abstract: Objective: The active plant ingredients have been successfully used in modern medicine to control appetites and energy hemostasis.This study designed to evaluate the efficacy of phytohormone methyl jasmonate (MJ) on foodrelated behaviors in rats.Methods: The adult male Wistar rats were randomly divided into different groups (7 rats), and infused intracerebroventriculary (i.c.v.) with MJ vehicle (DMSO) or MJ (2.5, 5 and 10 µg/rat).Then, the individual rats were placed in an automated open filed like apparatus to assess a 12-hour food-related activities in light and dark times.After behavioral tests, immunofluorescence staining of orexin 1 receptor (Orx1R) was studied in rats' hypothalamus.Results: MJ (2.5, 5 and 10 µg/rat) administration significantly decreased food intake in light and dark phases as compared to control group.Moreover, all the MJ -treated groups rats exhibited a decrease in visit to food container in light and dark times (p<0.001).In addition, rats infused with MJ at 5 µg, and 10 µg spent less time in the ports of food container in light and dark phases in comparison with control rats.Time in zone-related to food, and the locomotor activity were significantly decreased in MJ (5 µg) groups during light time, and in all MJ-injected groups in the dark time.Moreover, hypothalamic expression of Orx1R in rats-treated with MJ (5 µg) significantly lower as compared to control group.Conclusion: Overall, the results indicated a potential of MJ to modulate feeding-related behavior, and Orx1R expression in hypothalamus in rats.
Language: English
Subfield: Endocrine and Autonomic Systems
Field: Neuroscience
Domain: Life Sciences
Open Access status: diamond
Funder: University of Jiroft
OpenAlex ID: W4319600240