Year: 2023
Type: article
Abstract: Extracellular matrix (ECM) proteins containing cell‐attachable Arg‐Gly‐Asp (RGD) sequences exhibit variable bridging and non‐bridging in fibronectin‐collagen and laminin‐collagen complexes that can regulate inflammation, tissue repair, and wound healing. In this study, linking molecule‐mediated conjugation of 1D magnetic nanocylinders (MNCs) to material surfaces pre‐decorated with gold nanospheres (GNSs) is performed, thereby yielding RGD‐coated MNCs (RGD‐MNCs) over RGD‐coated GNSs (RGD‐GNSs) in a non‐bridging state. The RGD‐MNCs are drawn closer to the RGD‐GNSs via magnetic field‐mediated compression of the linking molecules to establish the bridging between them. Relative proportion of the RGD‐MNCs to the RGD‐GNSs is optimized to yield effective remote stimulation of integrin binding to variably bridged RGDs similar to that of invariably bridged RGDs used as a control group. Remote manipulation of the RGD bridging facilitates the attachment structure assembly of macrophages that leads to pro‐healing/anti‐inflammatory phenotype acquisition. In contrast, the non‐bridged RGDs inhibited macrophage attachment that acquired pro‐inflammatory phenotypes. The use of various nanomaterials in constructing heterogeneous RGD‐coated materials can further offer various modes in remote switching of RGD bridging and non‐bridging to understand dynamic integrin‐mediated modulation of macrophages that regulate immunomodulatory responses, such as foreign body responses, tissue repair, and wound healing.
Source: Advanced Functional Materials
Authors: Seong Yeol Kim, Ramar Thangam, Nayeon Kang, Hyunsik Hong, Chowon Kim, Sungkyu Lee, Subin Son, Hyun‐Jeong Lee, Kyong‐Ryol Tag, Sunhong Min, Daun Jeong, Jangsun Hwang, Kanghyeon Kim, Dahee Kim, Yuri Kim, Jinmyoung Joo, Bong Hoon Kim, Yangzhi Zhu, Sung‐Gyu Park, Hyun‐Cheol Song, Wujin Sun, Jae‐Pyoung Ahn, Woo Young Jang, Ramasamy Paulmurugan, Hong‐Kyu Kim, Jong Seung Kim, Heemin Kang
Institutions: Korea University, Korea Institute of Science and Technology, Korea University Anam Hospital, Ulsan National Institute of Science and Technology, Daegu Gyeongbuk Institute of Science and Technology, Terasaki Foundation, Korea Institute of Materials Science, Sungkyunkwan University, Virginia Tech, Stanford Medicine, Stanford University
Language: English
Subfield: Immunology and Allergy
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
Awards: G2079073928, G2245042858, G7523888510
OpenAlex ID: W4319785783