Lepidium sativumL. seeds extract as a preventive agent in experimentally induced urolithiasis model
Year: 2022
Type: article
Abstract: Lepidium sativum L. is an annual herb, belonging to Brassicaceae family and is used as a diuretic, antioxidant and nephroprotective agent.In the present study, we investigated the antiurolithiatic activity of aqueous and ethanolic extract of L. sativum (LS) seeds in 0.75% ethylene glycol (EG) and 1% ammonium chloride (AC) induced urolithias is model in rats.An in vitro assay was used to determine whether both extracts of LS prevent the formation of calcium oxalate (CaOx) or promote CaOx dissolution.Animals model of calcium oxalate urolithias was induced in the rat by adding EG 0.75% and 1% AC in drinking water.Aqueous extract of L. sativum (AELS) and ethanolic extract of L. sativum (EELS) orally at 100 and 200 mg/kg and cystone 750 mg/kg were administered along with EG and AC for 14 days (from 8th to 21st days).Various urine, serum and kidney parameters were assessed at the end of the study.The ethylene glycol group had a significant increase in levels of urine oxalate, calcium and phosphate, serum creatinine, urea and uric acid and renal tissues oxalates, as compared to normal.Histopathology shows significant changes.The treatment of AELS and EELS at 100 and 200 mg/kg doses, significantly decreased the urine oxalate, calcium, phosphate, serum creatinine, urea, anduric acid levels, in EG+ACinduced urolithiasis after 21 days.From the above results, it can be concluded that both extracts of L. sativum seeds have a significant inhibitory effect on calcium oxalate urolithiasis due to their diuretic and antioxidant activity.
Language: English
Subfield: Urology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4320031726