Work

A Novel Enzyme Biosensor Based on Ag/Reduced Graphene Oxide/Chitosan Membrane with Potentiometer for Pesticide Detection

Year: 2023

Type: article

Abstract: Long-term accumulation of pesticides in the environment to human and animal health. Acetylcholinesterase (AChE) biosensors with highly sensitive potentiometer transducers based on the membranes of Ag, reduced graphene oxide (rGO), and chitosan (CS) has been successfully developed. The membrane was made with a composition of 0.5 mM AgNO3, 2.5 mg/mL rGO, and 2% (w/v) CS coated on the surface of the Au electrode. The composition of the membrane with three ratios, namely 1:1:2, 2:1:3, and 3:1:4. Then, membrane Ag/rGO/CS and the enzyme AChE were immobilized on the membrane surface. The prepared biosensor has excellent conductivity, catalytic activity, and biocompatibility attributed to the synergistic effect of Ag/rGO/CS and glutaraldehyde (GTA) as crosslinkers and providing a hydrophilic surface for AChE adhesion. The linear range in biosensors is 1 × 10-8 to 1 µg L-1 with a regression coefficient of 0.9803 for 1:1:2 membrane, 0.9836 for 2:1:3 membrane, and 0.9850 for 3:1:4 membrane. The LOD is about 1 × 10-7 µg L-1 for all membranes. In addition, the biosensor showed good sensitivity, acceptable reproducibility, and stability, having an RSD of less than 5%. This biosensor makes it possible to provide a new and promising tool for analyzing pesticides, especially organophosphates.

Source: Molekul

DOI: 10.20884/1.jm.2023.18.1.6864

Authors: Mashuni Mashuni, Nur Arfa Yanti, Irnawati Irnawati, Fitri Handayani Hamid, Muhammad Jahiding

Institution: Universitas Halu Oleo

Language: English

Topic: Electrochemical sensors and biosensors

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $81

Funder: Kementerian Pendidikan, Kebudayaan, Riset, dan Teknologi