Supplementary figure 1 from Secondary Tumors Arising in Patients Undergoing BRAF Inhibitor Therapy Exhibit Increased BRAF–CRAF Heterodimerization
Year: 2023
Type: supplementary-materials
Abstract: Proximity Ligation Assay principle. Two primary antibodies recognize the two antigens BRAF and CRAF. Specific secondary antibodies (Probes) bind to the primary antibodies and are bound to a unique short DNA. When the PLA probes are in close proximity, the DNA strands can interact. After joining they are amplified via rolling circle amplification (RCA) using a polymerase. The signal is easily made visible as a distinct brown or fluorescent dot after enzymatic conversion of a substrate.
Authors: Lise Boussemart, Isabelle Girault, Hélène Malka-Mahieu, Christine Mateus, Emilie Routier, Margot Rubington, Nyam Kamsu-Kom, Marina Thomas, Gorana Tomasic, Sandrine Agoussi, Marie Breckler, Mélanie Laporte, Ludovic Lacroix, Alexander M. Eggermont, Andrea Cavalcanti, Florent Grange, Julien Adam, Stéphan Vagner, Caroline Robert
Language: English
Topic: Melanoma and MAPK Pathways
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4361237482