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Supplementary Figure 2 from Oncogenic Function of SCCRO5/DCUN1D5 Requires Its Neddylation E3 Activity and Nuclear Localization

Year: 2023

Type: supplementary-materials

Abstract: PDF file - 840K, A, Western blot analysis of lysates of NIH-3T3 cells stably transfected with vector alone, SCCRO5, SCCRO5_∆1-10 or SCCRO5_D195N/A219R/D225N and probed with antibody against SCCRO5 or tubulin as a loading control. SCCRO5_∆1-10 is not detected as the antibody recognizes an epitope that included this region. B, Bar graph depicting results of qRT-PCR on the same cells as in A showing expression of transgenes.

DOI: 10.1158/1078-0432.22450385.v1

Authors: Claire C. Bommeljé, Víola B. Weeda, Guochang Huang, Kushyup Shah, Sarina Bains, Elizabeth Buss, Manish Shaha, Mithat Gönen, Ronald Ghossein, Suresh Y. Ramanathan, Bhuvanesh Singh

Language: English

Topic: Ubiquitin and proteasome pathways

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

OpenAlex ID: W4361908249

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