Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat
Year: 2023
Type: review
Abstract: Genomics and genome editing promise enormous opportunities for crop improvement and elementary research. Precise modification in the specific targeted location of a genome has profited over the unplanned insertional events which are generally accomplished employing unadventurous means of genetic modifications. The advent of new genome editing procedures viz; zinc finger nucleases (ZFNs), homing endonucleases, transcription activator like effector nucleases (TALENs), Base Editors (BEs), and Primer Editors (PEs) enable molecular scientists to modulate gene expressions or create novel genes with high precision and efficiency. However, all these techniques are exorbitant and tedious since their prerequisites are difficult processes that necessitate protein engineering. Contrary to first generation genome modifying methods, CRISPR/Cas9 is simple to construct, and clones can hypothetically target several locations in the genome with different guide RNAs. Following the model of the application in crop with the help of the CRISPR/Cas9 module, various customized Cas9 cassettes have been cast off to advance mark discrimination and diminish random cuts. The present study discusses the progression in genome editing apparatuses, and their applications in chickpea crop development, scientific limitations, and future perspectives for biofortifying cytokinin dehydrogenase, nitrate reductase, superoxide dismutase to induce drought resistance, heat tolerance and higher yield in chickpea to encounter global climate change, hunger and nutritional threats.
Source: Frontiers in Genetics
Institutions: Guru Gobind Singh Indraprastha University, University of Allahabad, Indian Agricultural Research Institute, Birla Institute of Technology and Science, Pilani, Gautam Buddha University, ICAR-National Bureau Of Plant Genetic Resources, Amity University, Department of Biotechnology, National Institute of Plant Genome Research, Cairo University, International Center for Agricultural Research in the Dry Areas
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $3,295
Funders: International Center for Agricultural Research in the Dry Areas, Department of Biotechnology, Ministry of Science and Technology, India, Department of Science and Technology, Ministry of Science and Technology, India, Amity University, Indian Council of Agricultural Research, Cairo University, Indian Agricultural Research Institute, Science and Engineering Research Board
OpenAlex ID: W4366290956