Year: 2023
Type: article
Abstract: SR proteins are conserved RNA‐binding proteins best known as splicing regulators that have also been implicated in other steps of gene expression. Despite mounting evidence for a role in plant development and stress responses, the molecular pathways underlying SR protein regulation of these processes remain poorly understood. Here we show that the plant‐specific SCL30a SR protein negatively regulates ABA signaling to control seed traits and stress responses during germination in Arabidopsis . Transcriptome‐wide analyses revealed that loss of SCL30a function barely affects splicing, but largely induces ABA‐responsive gene expression and genes repressed during germination. Accordingly, scl30a mutant seeds display delayed germination and hypersensitivity to ABA and high salinity, while transgenic plants overexpressing SCL30a exhibit reduced ABA and salt stress sensitivity. An ABA biosynthesis inhibitor rescues the enhanced mutant seed stress sensitivity, and epistatic analyses confirm that this hypersensitivity requires a functional ABA pathway. Finally, seed ABA levels are unchanged by altered SCL30a expression, indicating that the gene promotes seed germination under stress by reducing sensitivity to the phytohormone. Our results reveal a new player in ABA‐mediated control of early development and stress response.
Source: Plant Cell & Environment
DOI: 10.1111/pce.14593
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: hybrid
Estimated APC paid: $5,050
Awards: ROLE OF ALTERNATIVE SPLICING IN THE UBIQUITYLATION PATHWAY AND STRESS-RELATED RESPONSES IN ARABIDOPSIS THALIANA, Um Fator de "Splicing" que Regula o Tamanho e a Resposta ao Stress da Semente, G5454278309, Caracterização molecular de uma nova proteína de ligação ao ARN que confere resistência a stress hídrico e salino durante a germinação, GREEN-IT "Biorecursos para a Sustentabilidade"
OpenAlex ID: W4366998370