MLPK function is not required for self-incompatibility in the S29 haplotype of Brassica rapa L.
Year: 2023
Type: article
Abstract: Key message S29 haplotype does not require the MLPK function for self-incompatibility in Brassica rapa. Abstract Self-incompatibility (SI) in Brassicaceae is regulated by the self-recognition mechanism, which is based on the S-haplotype-specific direct interaction of the pollen-derived ligand, SP11/SCR, and the stigma-side receptor, SRK. M locus protein kinase (MLPK) is known to be one of the positive effectors of the SI response. MLPK directly interacts with SRK, and is phosphorylated by SRK in Brassica rapa. In Brassicaceae, MLPK was demonstrated to be essential for SI in B. rapa and Brassica napus, whereas it is not essential for SI in Arabidopsis thaliana (with introduced SRK and SP11/SCR from related SI species). Little is known about what determines the need for MLPK in SI of Brassicaceae. In this study, we investigated the relationship between S-haplotype diversity and MLPK function by analyzing the SI phenotypes of different S haplotypes in a mlpk/mlpk mutant background. The results have clarified that in B. rapa, all the S haplotypes except the S29 we tested need the MLPK function, but the S29 haplotype does not require MLPK for the SI. Comparative analysis of MLPK-dependent and MLPK-independent S haplotype might provide new insight into the evolution of S-haplotype diversity and the molecular mechanism of SI in Brassicaceae.
Source: Plant Reproduction
Authors: Mayu Ohata, Yoshinobu Takada, Yui Sato, Takumi Okamoto, Kohji Murase, Seiji Takayama, Go Suzuki, Masao Watanabe
Language: English
Topic: Plant Reproductive Biology
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,860
Awards: アブラナ科植物の自家および種間不和合性経路の統合的理解, G2279820041, Genetic analysis of the pollen-stigma incompatibility in Brassica rapa., ５％炭酸ガスによらず自殖を可能にする鍵因子解析を用いた環境調和型採種作物生産技術, Molecular basis reconstruction of evaluation system of pollination responses for diversification of breeding materials in cruciferous crops, アブラナ近郊系統間に生じた新規一側性不和合性の遺伝育種学的解析, G570906089, Integrated understanding of interspecific-incompatibility and self-incompatibility in the Brassicaceae, G6857536765, Interdisciplinary research on the use of historical and archaeological materials to build a diversity of crop resources for the next generation.
OpenAlex ID: W4367047925