Characteristics of the Fire Blight Pathogen Erwinia Amylovora Isolated From Fruit Cultures in Southeastern Kazakhstan
Year: 2023
Type: article
Abstract: One of the most dangerous and quarantine diseases of fruit crops of the Rosaceae family is fire blight which affected on plants. Its causative agent Erwinia amylovora causes necrosis of all organs of host plants. In the territory of Republic of Kazakhstan, the disease belongs to a limited number of quarantine species. A special place is given to laboratory diagnostics of E.amylovora, which is necessary for reliable detection of the pathogen. Bacteriological analyses of diseased samples of fruit crops were carried out under laboratory conditions to isolate pathogens into pure cultures. Isolating of the quarantine and causative agent of fire blight into a pure culture was carried out on King B nutrient media, Levan and Miller Schroth selective media. They were tested for pathogenicity on indicator plants of indoor geranium by hypersensitivity reaction by the infectious-infiltration method of Clement. The pathogenicity of isolated bacteria was also tested by testing on unripe pear fruits, on which the damaging bacteria caused the release of milky-white exudate. After the selected isolates of the fire blight pathogen were identified by two pairs of specific primers PEANT F/PEANT2 R and FER F/rgER2-R approved by the European and Mediterranean Plant Protection Organization. For molecular differentiation, the nucleotide sequences of 16S rRNA genes were compared
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: hybrid
OpenAlex ID: W4378419862