In vitro antioxidant and wound healing activity of Sargassum polycystum hydroethanolic extract in fibroblasts and keratinocytes
Year: 2023
Type: article
Abstract: Objective: To investigate the in vitro antioxidant and wound healing properties of the hydroethanolic extract of Sargassum polycystum , and elucidate the mechanism of its wound healing activity. Methods: Human dermal fibroblast and HaCaT cells were used to evaluate the proliferation by sulforhodamine B and dsDNA assay after treatment with Sargassum polycystum extracts. Scratch wound healing and phalloidin-rhodamine staining were employed to observe migratory activity and filopodia formation, respectively. Western blot and real-time RT-PCR assays were performed to determine the protein and gene expressions related to wound healing activities. Results: The phytochemical analysis found a higher level of flavonoid than phenolic compound in Sargassum polycystum extracts. In human dermal fibroblast cells, Sargassum polycystum extracts at 50 and 100 µg/mL significantly increased fibroblast proliferation and the gene expressions of hyaluronic acid synthase 1 ( HAS1 ), HAS2 , HAS3 , collagen type 1 alpha 1 chain ( COL1A1 ), collagen type 3 alpha 1 chain ( COL3A1 ), and elastin . The phosphorylation of Akt, ERK1/2, and p38 MAPK was also significantly upregulated after treatment with Sargassum polycystum extracts. Additionally, 50 and 100 µg/mL of the extracts prominently enhanced the proliferation, migration, and filopodia formation of HaCaT cells, as well as the protein levels of pFAK/FAK, pSrc/Src, pAkt/Akt, pERK1/2/ERK1/2, Rac1 and Cdc42. Conclusions: Sargassum polycystum extracts show promising wound healing activities in human dermal fibroblasts and keratinocytes.
Institutions: Prince of Songkla University, Naresuan University
Language: English
Subfield: Aquatic Science
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4378652192