Establishing SARS-CoV-2 membrane protein-specific antibodies as a valuable serological target via high-content microscopy
Year: 2023
Type: article
Study design: Observational Study
Abstract: The prevalence and strength of serological responses mounted toward SARS-CoV-2 proteins other than nucleocapsid (N) and spike (S), which may be of use as additional serological markers, remains underexplored. Using high-content microscopy to assess antibody responses against full-length StrepTagged SARS-CoV-2 proteins, we found that 85% (166/196) of unvaccinated individuals with RT-PCR confirmed SARS-CoV-2 infections and 74% (31/42) of individuals infected after being vaccinated developed detectable IgG against the structural protein M, which is higher than previous estimates. Compared with N antibodies, M IgG displayed a shallower time-dependent decay and greater specificity. Sensitivity for SARS-CoV-2 seroprevalence was enhanced when N and M IgG detection was combined. These findings indicate that screening for M seroconversion may be a good approach for detecting additional vaccine breakthrough infections and highlight the potential to use HCM as a rapidly deployable method to identify the most immunogenic targets of newly emergent pathogens.
Source: iScience
Authors: Daniel M. Williams, Hailey R. Hornsby, Ola M. Shehata, Rebecca Brown, Marta Gallis, Naomi Meardon, Thomas A.H. Newman, Megan Plowright, Domen Zafred, Amber S.M. Shun-Shion, Anthony J. Hodder, Deepa Bliss, Andrew Metcalfe, James R. Edgar, David E. Gordon, Jon R. Sayers, Martin J. Nicklin, Miles Carroll, Paul J. Collini, Stephen Brown, Thushan I. de Silva, Andrew A. Peden
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,150
Funders: University of Dundee, Wellcome Trust, UK Research and Innovation, National Institute for Health and Care Research, British Heart Foundation, Department of Health and Social Care, Royal Society, European Commission, University of Sheffield, Sheffield Teaching Hospitals NHS Foundation Trust, UK Coronavirus Immunology Consortium, Horizon 2020 Framework Programme, Biotechnology and Biological Sciences Research Council, Horizon 2020, H2020 Marie Skłodowska-Curie Actions
Awards: Development of Kinetoplastida Flap Endonuclease Inhibitors in Search for Novel Therapeutics, BB/J014443/1, Investigation into OPG and related biomarkers in incident cases of pulmonary hypertension, Tethered exosomes and their role in MHC-II presentation, Elucidating the role of SNAREs in membrane contact site formation and cholesterol homeostasis, G37668656, G3968328659, G434337789, G460868686, Development of Kinetoplastida Flap Endonuclease Inhibitors in Search for Novel Therapeutics, Immunogenicity of intranasal live attenuated influenza vaccination (LAIV) and bidirectional interactions with the host microbiome., G4940775716, G5474507064, Immunogenicity of intranasal live attenuated influenza vaccination (LAIV) and bidirectional interactions with the host microbiome., G6868616705, G8338067429, Defining the molecular mechanisms of the OPG-FAS- TRAIL axis in pulmonary arterial hypertension
OpenAlex ID: W4379745679