O51 | Algal extracts as potential anticancer agents: Preliminary investigations in C2 canine mastocytoma cell line
Year: 2023
Type: conference-abstract
Abstract: ABSTR ACT additionally granzyme B and programmed death (PD)-1 for activated canine T-cells.Results: IBA-1 + and CD163 + immunosuppressive macrophages were localized in the stroma surrounding the tumor mass in the canine and feline TME, and MHCII + antigen-presenting cells were identified as most abundant immune cells in these stromal areas.For both species, the number of these immune cells was significantly higher in grade 3 compared to grade 1 and 2 mammary tumors, indicative for an enhanced immunosuppressive leukocyte reaction upon tumor progression.Moreover, IBA-1 + and CD163 + macrophages were also significantly more present in grade 3-associated SLNs, suggesting immunosuppression at this metastatic site upon advanced disease stages.Immunosuppression in the canine mammary TME was further characterized based on the location of granzyme B + and PD-1 + activated T-cells.Remarkably, despite the prominent CD3 + T-cell influx, PD-1 and granzyme B stainings remained either scattered throughout or restricted to CD20 + B-cell-rich islands in the TME stroma, rendering tumor-infiltrated CD3 + T-cells inactive.Conclusions: Our results highlight the immunosuppressive nature of the canine and feline mammary TME, which is highly relevant for translatable studies that can ultimately benefit both companion animals themselves and human patients.
DOI: 10.1111/jvp.13217
Authors: Mucignat G., Lakhdar F., Lucatello L., Capolongo F., Pauletto M., Giantin M., Dacasto M.
Language: English
Subfield: Aquatic Science
Domain: Life Sciences
Open Access status: bronze
Funder: Ministero della Salute
Award: G5036270952
OpenAlex ID: W4380231020