Work

Electrochemical Detection of Single Nucleotide Polymorphism in Short DNA Sequences Related To Cattle Gene

Year: 2012

Type: article

Abstract: Electrochemical nucleic acid biosensor for detection of single nucleotide polymorphism (SNP) in Fatty acid binding protein 4 (FABP4) has been developed by immobilization of single stranded thiolated probe DNA (ss-DNA-SH) onto gold surfaces (ss-DNA-SH/Au) and its hybridization with wild and mutant complementary sequences related to Korean cattle FABP4 gene in the presence methylene blue. The immobilization of ss-DNA-SH onto gold surfaces was characterized by atomic force microscopy (AFM), Fourier transforms infrared microscopy (FT-IR), cyclic voltammetric (CV) and impendence techniques. The SNP in Korean cattle FABP4 gene was detected by measuring the methylene blue accumulation on ss-DNA-SH/Au electrode hybridized with wild (wild-ds-DNA- SH/Au) and mutant (mutant-ds-DNA-SH/Au) complementary sequences using CV technique. The sensitivities of wild-ds-DNA-SH/Au and mutant-ds-DNA-SH/Au electrodes are 0.0164 μA/ng μL−1 with 0.998 regression coefficient (R) and 0.0122 μA/ng μL−1 with 0.993 R, respectively. The ssDNA- SH/Au electrode can detect the wild and mutant type complementary targets related to FABP4 gene in the range of 5-10 ng μL−1.

Source: International Journal of Electrochemical Science

DOI: 10.1016/s1452-3981(23)16926-4