Work

Nanostructured Titanium Oxide Platform for Application to Ascorbic Acid Detection

Year: 2012

Type: article

Abstract: Nanostructured TiO2 films have been deposited onto indium tin oxide (ITO) films by sol–gel technique to immobilize ascorbate oxidase (AsOx) onto nanostructured TiO2 films. The TiO2/ITO matrix, AsOx/Nano-TiO2/ITO bioelectrodes have been characterized using spectroscopic techniques and used to fabricate ascorbic acid biosensor. Nanostructured TiO2 based ascorbic acid biosensor has a response time about 20 s, an apparent Km (Kmapp) value of 30.80mg/dL (1.75 mM) and can be used to estimate ascorbic acid concentration up to 200 mg dL−1. The AsOx/Nano-TiO2/ITO bioelectrodes have a detection limit of 50 mg dL−1 with sensitivity of 0.212 mA/mg dL cm2. The enzyme films were found to be thermally stable up to 40 °C and have a shelf-life of about 4 weeks when stored at 4 °C.

Source: International Journal of Electrochemical Science

DOI: 10.1016/s1452-3981(23)19631-3

Authors: P.M. Chavhan, Venu Reddy, CheolGi Kim

Institution: Chungnam National University

Language: English

Topic: Electrochemical sensors and biosensors

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $1,670

Funders: World Institute of Kimchi, National Research Foundation of Korea, Ministry of Education, Science and Technology