In vitro and in vivo Antidiabetic Effect of the Aqueous Extract of Garlic (Allium sativum L.) Compared to Glibenclamide on Biochemical Parameters in Alloxan-induced Diabetic Mice
Year: 2023
Type: article
Abstract: Diabetes mellitus (DM) is the most common chronic disease.Scientific research has turned to traditional herbal remedies as a potential adjunct therapy to maintain better glycemic control with minimal side effects.Garlic has been known since ancient times as an essential regulator either in diet or in medicine.Today the use of garlic is growing and widespread all over the world.This study is carried out to evaluate in vivo and in vitro antidiabetic effects of garlic extract compared to glibenclamide on biochemical parameters in alloxan induced diabetic mice.In-vitro antidiabetic effect of the A. sativum aqueous extract was conducted using αamylase assay.Meanwhile in-vivo antidiabetic activity was conducted using alloxan induced diabetic mice with an intraperitoneal injection of 160 mg/kg body weight.The diabetic mice were divided into five groups, two of which were given garlic extract orally (200 mg/kg and 400 mg/kg) and a group composed of diabetic mice were given the standard drug, glibenclamide, orally at a dose of 2.5 mg/kg.The control mice (normal and diabetic) were fed normal saline once a day for 28 days.The preventive effect of garlic extract was proved to have the same result as the standard drug, glibenclamide when given at the dose indicated above.This result was shown when experiments dealt with blood glucose, glycosylated hemoglobin levels, total cholesterol, triglycerides, total lipids, alanine aminotransferase (ALAT), and aspartate aminotransferase (ASAT), with significant increases in plasma insulin.In addition, the plant extract exhibited a considerable inhibitory effect on α-amylase activity with IC50 value of 680.54± 0.58μg/ml.The present study shows that garlic extract possesses significant, potent anti-diabetic activity in vitro and in vivo, and that this activity is dose related.The garlic extract also recovers metabolic alterations and preserves insulin secretion capacity.
Language: English
Topic: Garlic and Onion Studies
Subfield: Plant Science
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4383372544