Work

Type 2–resistant starch and Lactiplantibacillus plantarum NCIMB 8826 result in additive and interactive effects in diet-induced obese mice

Year: 2023

Type: article

Abstract: Little is known about how combining a probiotic together with a prebiotic dietary fiber affects the ability of either biotic to improve health. We hypothesized that prebiotic, high-amylose maize, type 2 resistant starch (RS) together with probiotic Lactiplantibacillus plantarum NCIMB8826 (LP) as a complementary synbiotic results in additive effects on the gut microbiota in diet-induced obese (DIO) mice and other body sites. DIO C57BL/6J male mice were fed a high-fat (HF) diet adjusted to contain RS (20% by weight), LP (109 cells every 48 h), both (RS+LP) for 6 weeks. As found for mice fed RS, cecal bacterial alpha-diversity was significantly reduced in mice given RS+LP compared to those fed LP and HF controls. Similarly, both RS+LP and RS also conferred lower quantities of cecal butyrate and serum histidine and higher ileal TLR2 transcript levels and adipose tissue IL-6 protein. As found for mice fed LP, RS+LP fed mice had higher colonic tissue TH17 cytokines, reduced epididymal fat immune and oxidative stress responses, reduced serum carnitine levels, and increased transcript quantities of hepatic carnitine palmitoyl transferase 1α. Notably, compared to RS and LP consumed separately, there were also synergistic increases in colonic glucose and hepatic amino acids as well antagonistic effects of LP on RS-mediated increases in serum adiponectin and urinary toxin levels. Our findings show that it is not possible to fully predict outcomes of synbiotic applications based on findings of the probiotic or the prebiotic tested separately, and therefore studies should be conducted to test new synbiotic formulations.

Source: Nutrition Research

DOI: 10.1016/j.nutres.2023.07.003