Multi-region spatial transcriptome analysis reveals cellular networks and pathways associated with hepatocellular carcinoma recurrence
Year: 2023
Type: preprint
Study design: Observational Study
Abstract: Hepatocellular carcinomas (HCC) are driven by various etiologies and molecular diversity at presentation. Patient prognosis post-surgery is generally dismal, and the majority respond poorly to adjuvant targeted and/or immuno-therapies. Tumours are an ecosystem comprised of organization and interaction between different cell types that may contribute to clinically significant outcomes, such as disease recurrence. To better understand this phenomenon, we leveraged on a local cohort of patients with or without recurrence to generate spatial transcriptome profiles from multiple sectors from each tumour. We identified widespread gene expression intra- and inter tumour heterogeneity observed across the tumour sectors. Our analysis also revealed the cell type enrichment and localization, and ligand-receptor interactions identify a specific subset of endothelial cell enriched in primary tumours of patients with recurrence. Altogether, this study describes the spatial gene expression landscape in HCC patients associated with disease recurrence.
Authors: Aziz Aiderus, Pratap Veerabrahma Seshachalam, Khaireen Idzham, Matias Caldez, Raghuvaran Shanmugam, Ita Novita Sari, Dorcas Hei Hui Ying, Shay Lee Chong, Karthik Sekar, Sin Chi Chew, Gao Bin Chen, Alexander Yaw-Fui Chung, Peng Chung Cheow, Juinn Huar Kam, Alfred Wei-Chieh Kow, Iyer Shridhar Ganpathi, Shihleone Loong, Wei-Qiang Leow, Kaina Chen, Rawisak Chanwat, Vanessa H. de Villa, Peng Soon Koh, Glenn K Bonney, Brian K. P. Goh, Wai Leong Tam, Vinay Tergaonkar, Pierce Kah Hoe Chow
Institutions: Agency for Science, Technology and Research, Institute of Molecular and Cell Biology, National Cancer Centre Singapore, National University of Singapore, Singapore General Hospital, SingHealth Duke-NUS Academic Medical Centre, Duke-NUS Medical School, Sengkang General Hospital, National University Health System, National Cancer Institute of Thailand, The Medical City Ortigas, University of Malaya, Genome Institute of Singapore
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4385312401