Extraction and characterization of sericin obtained from unreelable cocoons of Bombyx mori L
Year: 2023
Type: article
Abstract: Silkworm, Bombyx mori L. cocoon is a protective covering comprising of two important proteins viz., sericin and fibroin. Sericin, a water-soluble protein representing about 30% of the cocoon weight is perse dissolved by cooking to unwind the silk filament from the cocoon during reeling. In the present study, the sericin was extracted from cut cocoons. Extraction was carried out by two methods viz., open boiling and autoclave method at different time regimes viz., 30 min, 60 min and 90 min. The recovery of crude sericin powder was done by hot air drying and freeze-drying. Maximum recovery (14.12 ± 0.05 %) of crude sericin powder was obtained when extraction was carried out by autoclave method and drying by lyophilization. The sericin samples were studied for qualitative, quantitative and biological parameters. Extraction by autoclave method showed maximum protein content and protein purity as compared to open boiling method. Maximum gelation was observed in the samples extracted for 60 min followed by 30 min time duration. Differential molecular weight protein bands were observed at three-time durations. Molecular weight ranging from 15-25 KDa was obtained in the sample extracted for 30 min and 60 min. While as, reduced molecular weight bands of 10-15 KDa size was obtained when extraction was carried for 90 min. The sericin showed concentration dependent antioxidant activity to the tone of 64 ± 7 % at 40 mg/mL concentration. Sericin did not show any antibacterial and antifungal activity.
Authors: Aabid Bhat, Aabid Tantray, Phalisteen Sultan, Mir Maqbool, Sadaqat farooq, lubna Altaf, khursheed Sahaf, irfan khan
Language: English
Subfield: Biomaterials
Field: Materials Science
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W4385463189