Pb1818: Prognostic Value of Neoplastic Inflammation and Cytokines in the Course of Acute Myeloid Leukemia With Maturation
Year: 2023
Type: conference-abstract
Study design: Observational Study
Abstract: Topic: 3. Acute myeloid leukemia - Biology & Translational Research Background: Course of acute myeloblastic leukemia (AML) with maturation, subtype M2 (AML M2); (FAB 2016), has an individual behavior and may depend on the an indicators of neoplastic inflammation, leukocytosis/cytopenia, lymphocytosis, blasts, cytokines in the peripheral blood (PB) of AML M2 patients (pts). This was confirmed by the anti-inflammatory effect of glucocorticoids, which improves the effectiveness of AML treatment. In addition, there are few reports on the role and indicators of neoplastic inflammation in the course of AML M2. Aims: To determine the prognostic value of the an indicators of neoplastic inflammation and cytokines, TNF and TGFβ1, in the course and survival of AML M2 pts. Methods: We examined of 14 untreated AML M2 pts; median age 68,5 (range 47 – 76) years and 10 AML pts without maturation (ALM M1) as the compared group (median age 63, range 26 – 81 years). The control group consisted of 15 healthy individuals. All AML pts (n=24) had blasts in PB (60.90 ± 7.78) ×10 9/l. The concentration of TNF and TGFβ1, were detected in the plasma and 24-h supernatants of peripheral blood mononuclear cells mixed with blasts (PBMC), by biological methods. Indicators of inflammation were detected in the hemograms of AML pts. The statistical processing were determined in the Exel program. Results: The AML M2 pts had a higher number of blasts, leukocytes and a lower content of mature neutrophils and lymphocytes in the hemogram, than in AML M1 pts. It could affect on the PBMC production of cytokines and the survival of AML pts. The concentration of TNF in the supernatants of PBMC of AML M2 pts was significantly higher than in AML M1 and control in both cases; p˂0.001. Detected a direct correlation between the TNF in the plasma of AML M2 pts and the number of blasts (r= 0.48) and an inverse correlation between the number of blasts and the TGFβ1 in the plasma (r= - 0, 36), as well as with the concentration of the TGFβ1 in the supernatants of PBMC (r= - 0.59) of the AML M2 pts. The median survival of AML M2 pts was three times shorter than in the AML M1 pts. An inverse correlation was established between survival and TNF plasma of AML M2 pts (r= - 0.28). To compare, the survival of AML M1 pts was positively correlated both of TNF and TGF in plasma r= 0.92 and r= 0.42, respectively. Summary/Conclusion: The indicators of neoplastic inflammation, cytokines, TNF, TGFβ1, have different pathogenetic effects on the survival both of AML M2 and AML M1 pts. In addition, they not only identify of AML M2 pts as a high-risk group, but provide well-defined prognostic markers for future clinical trials and evaluation of survival in AML pts too. Keywords: Acute myeloid leukemia, Cellular immunity, Cytokine, Prognostic
Source: HemaSphere
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,480
OpenAlex ID: W4385699050