Pb1994: Comparison of Patients With Myelodysplastic Syndromes With High and Low Ogata Score Values on Cytometry in Relation to the Presence of Cytogenetic Abnormalities - a Preliminary Study
Year: 2023
Type: conference-abstract
Study design: Observational Study
Abstract: Topic: 9. Myelodysplastic syndromes - Biology & Translational Research Background: Myelodysplastic syndrome (MDS) is a group of diseases with abnormal and inadequate hematopoiesis. In MDS, we encounter single-, double- or triple-lineage cytopenia. In order to assess prognosis, the presence of poor prognostic cytogenetic abnormalities is taken into account, among other things. Thus, it is very important to select patients with an increased risk of an unfavorable course of the disease as soon as possible. Aims: The aim of the study was to compare patients with MDS who had low and high Ogata values. The Ogata score parameter with the presence or absence of any cytogenetic abnormalities in the bone marrow were evaluated. Methods: Bone marrow from 40 patients with MDS and positive Ogata score, including 17 patients with positive cytogenetic abnormality were examined. Cytogenetic studies and molecular evaluation of genetic abnormalities were performed using classical cytogenetics, FISH method and PCR reaction. The Ogata scores were analyzed using the multicolor flow cytometry method and referred to a specific cytometric algorithm. Results: In our study, we observed that patients with a high Ogata value also revealed cytogenetic abnormalities. In patients with a low Ogata value, we did not observe cytogenetic disorders. In patients with a high Ogata score value > 3 points we have shown cytogenetic changes. We didn’t observe cytogenetic abnormalities in any patients with Ogata score 0 point (p < 0.005). Summary/Conclusion: Our study confirmed that Ogata score, as a well-known flow cytometry algorithm developed for analyzing cells in patients with MDS, could be helpful in the diagnosis and risk stratification of MDS. Ogata score analysis identify patients with cytogenetic abnormalities and indicate high-risk patients. On this basis, in countries or centers around the world where there is less opportunity for cost-saving reasons to perform cytogenetic testing, high-risk patients in whom we suspect we may find cytogenetic abnormalities can be selected based on the Ogata score. The conclusions of our work could be of practical benefit in the daily work of a hematologist. The study is being further continued at our center. Keywords: MDS
Source: HemaSphere
Authors: Krzysztof Gawronski, Elżbieta Rutkowska, Iwona Kwiecień, Katarzyna Kulik, Alicja Dudzik, Agata Zakrzewska, Piotr Rzepecki
Institution: Wojskowy Instytut Medyczny
Language: English
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,480
OpenAlex ID: W4386102477