The evaluation of five serological assays in determining seroconversion to peste des petits ruminants virus in typical and atypical hosts
Year: 2023
Type: article
Abstract: Peste des petits ruminants (PPR) is an infectious viral disease, primarily of small ruminants such as sheep and goats, but is also known to infect a wide range of wild and domestic Artiodactyls including African buffalo, gazelle, saiga and camels. The livestock-wildlife interface, where free-ranging animals can interact with captive flocks, is the subject of scrutiny as its role in the maintenance and spread of PPR virus (PPRV) is poorly understood. As seroconversion to PPRV indicates previous infection and/or vaccination, the availability of validated serological tools for use in both typical (sheep and goat) and atypical species is essential to support future disease surveillance and control strategies. The virus neutralisation test (VNT) and enzyme-linked immunosorbent assay (ELISA) have been validated using sera from typical host species. Still, the performance of these assays in detecting antibodies from atypical species remains unclear. We examined a large panel of sera (n = 793) from a range of species from multiple countries (sourced 2015-2022) using three tests: VNT, ID VET N-ELISA and AU-PANVAC H-ELISA. A sub-panel (n = 30) was also distributed to two laboratories and tested using the luciferase immunoprecipitation system (LIPS) and a pseudotyped virus neutralisation assay (PVNA). We demonstrate a 75.0-88.0% agreement of positive results for detecting PPRV antibodies in sera from typical species between the VNT and commercial ELISAs, however this decreased to 44.4-62.3% in sera from atypical species, with an inter-species variation. The LIPS and PVNA strongly correlate with the VNT and ELISAs for typical species but vary when testing sera from atypical species.
Source: Scientific Reports
Authors: Matthew Tully, Carrie Batten, Martin Ashby, Mana Mahapatra, Krupali Parekh, Satya Parida, Felix Njeumi, Brian Willett, Arnaud Bataille, Genevieve Libeau, Olivier Kwiatek, Alexandre Caron, Francisco J. Berguido, Charles E. Lamien, Giovanni Cattoli, Gerald Misinzo, Julius Keyyu, Daniel Mdetele, Francis Gakuya, Sanne Charles Bodjo, Fatima Abdelazeem Taha, Husna Mohamed Elbashier, Abdelmalik Ibrahim Khalafalla, Abdinasir Y. Osman, Richard Kock
Institutions: The Pirbright Institute, Food and Agriculture Organization of the United Nations, MRC University of Glasgow Centre for Virus Research, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Université de Montpellier, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Animal, Santé, Territoires, Risques et Ecosystèmes, International Atomic Energy Agency, Southern African Centre for Infectious Disease Surveillance, Sokoine University of Agriculture, Tanzania Wildlife Research Institute, Ministry of Livestock and Fisheries, Wildlife Research and Training Institute, African Union, Central Veterinary Research Laboratory, University of Khartoum, Abu Dhabi Agriculture and Food Safety Authority, Royal Veterinary College, Wasaaradda Caafimaadka, National Institute of Health Somalia
Language: English
Topic: Virology and Viral Diseases
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,390
Awards: A One Health approach to pan-valent morbillivirus vaccines, ANIHWA CALL1:Improved Understanding of Epidemiology of PPR (IUEPPR), High Containment, ANIHWA CALL1:Improved Understanding of Epidemiology of PPR (IUEPPR), Atypical bovine morbillivirus infections in the Serengeti ecosystem, Pathway to Peste des Petits Ruminants Virus Elimination - methods for complex ecosystems, Recognition and control of virus infections, Development of multispecies validated serology protocols for complex ecosystems, focused on East Africa, in support of Global PPR eradication, Transmission and Epidemiology
OpenAlex ID: W4386560103