Visualization of trans homophilic interaction of clustered protocadherin in neurons
Year: 2023
Type: article
Abstract: Clustered protocadherin (Pcdh) functions as a cell recognition molecule through the homophilic interaction in the central nervous system. However, its interactions have not yet been visualized in neurons. We previously reported PcdhγB2-Förster resonance energy transfer (FRET) probes to be applicable only to cell lines. Herein, we designed γB2-FRET probes by fusing FRET donor and acceptor fluorescent proteins to a single γB2 molecule and succeeded in visualizing γB2 homophilic interaction in cultured hippocampal neurons. The γB2-FRET probe localized in the soma and neurites, and FRET signals, which were observed at contact sites between neurites, eliminated by ethylene glycol tetraacetic acid (EGTA) addition. Live imaging revealed that the FRET-negative γB2 signals rapidly moved along neurites and soma, whereas the FRET-positive signals remained in place. We observed that the γB2 proteins at synapses rarely interact homophilically. The γB2-FRET probe might allow us to elucidate the function of the homophilic interaction and the cell recognition mechanism.
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: hybrid
Funders: Ministry of Education, Culture, Sports, Science and Technology, National Institutes of Health, Japan Society for the Promotion of Science, Japan Science and Technology Agency, National Institute of Mental Health, National Institute for Physiological Sciences, Precursory Research for Embryonic Science and Technology
Awards: G1391210221, Partner selection of climbing fiber by Purkinje cell identity, Mechanism of generating cell assembly, Elucidation of the mechanism by which neural connections predetermined in cellular lineages are selectively affected by sensory input, Epigenomic Regulation of a Large, Neuron-specific Chromatin Domain, G2257467825, Advanced Bioimaging Support, G4529733334, G4607286875, 細胞内タンパク質局在を高解像度で可視化できるS/N比の良いゲノム編集マウスの開発, Advanced Bioimaging Support, Analysis of the molecular mechanisms of the neuroprotective effect of the neuropeptide PACAP in ischaemia.
OpenAlex ID: W4386590237