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MICROWAVE-ASSISTED EXTRACTION IMPACTS ON PHARMACOLOGICAL ACTIVITY OF Vernonia amygdalina Delile LEAF EXTRACTS AND FT-IR APPLICATION FOR PHYTOCHEMICALS ANALYSIS

Year: 2023
Type: article
Abstract: Vernonia amygdalina (VA) is a native plant from Africa that is widely distributed to Asia, especially in Indonesia.One of the factors that can affect the pharmacological activity of natural ingredients is the extraction process.This study aims to show the effect of extraction methods in the VA leaves extraction process on phytochemical content and their correlation with pharmacological activities.VA leaves extract in this study was obtained using different extraction methods.The extracts were investigated for total phenolic content (TPC) and total flavonoid content (TFC) using a calorimetry assay.Principal Component Analysis (PCA) was used to group the extracts based on FTIR data.Antioxidant activity of the extracts was done using DPPH and ABTS assay.While the cytotoxic activity of the extracts was carried out using an MTT assay on T47D, WiDr, and HeLa cells.The cytotoxic activity of extracts was described using the IC50 value.There were significantly different total phenolic content and total flavonoid content of extracts (p<0.05).The microwave-assisted extraction (MAE) extract was identified to have the highest TFC, DPPH, ABTS, and cytotoxic values of 11.06±0.77mg QE/g dry powder, 63.05±3.21µg/mL, 32.19±1.23 µg/mL, and 133.922±3.22µg/mL (T47D), 194.547±3.43µg/mL (WiDr), and 1275.436±11.42µg/mL (HeLa), respectively (p<0.05).This is supported by PCA analysis which shows that there are differences in extracts based on FTIR data.This study reports that each extract of VA leaves gives a distinct phytochemical profile (TPC, TFC, and FTIR spectrum) that contributes to the antioxidant activity and cytotoxic activity.
DOI: 10.31788/rjc.2023.1638330
Language: English
Subfield: Plant Science
Open Access status: diamond
OpenAlex ID: W4386901754
FWCI: 1.108
Cites: 39
Cited by: 8
Related to: 10