Work

Nakazawaea atacamensis f.a., sp. nov. a novel non-conventional fermentative ascomycetous yeast species from the Atacama Desert

Year: 2023

Type: preprint

Abstract: Atacama is the most hyper-arid Desert in the world. In this study, we describe a novel species, Nakazawaea atacamensis f. a., sp. nov., isolated from plant samples in the Atacama Desert of Chile. In total, three isolates of N. atacamensis were obtained from independent Neltuma chilensis bark samples (synonym Prosopis chilensis, Algarrobo). The novel species was delineated based on morphological, physiological, biochemical, and molecular characteristics. A neighbour-joining analysis using the sequences of the D1/D2 domains of LSU rRNA revealed that N. atacamensis sp. nov. clustered with Nakazawaea pomicola . The sequence of N. atacamensis differed from closely related species by 1.3% to 5.2% in the D1/D2 domains. A phylogenomic analysis based on single nucleotide polymorphism’s data confirms that the novel species belongs to the genus Nakazawaea, and placed N. atacamensis closer to N. peltata . Phenotypic comparisons demonstrated that N. atacamensis sp. nov. exhibited distinct carbon assimilation patterns compared to its related species. Genome sequencing of the ATA-11A-B T strain revealed a genome size of approximately 12.4 Mbp, similar to other Nakazawaea species, with 5,116 protein-coding genes annotated using InterProScan. In addition, N. atacamensis exhibited the capacity to ferment synthetic wine must, representing a potential new yeast for mono or co-culture wine fermentations. This comprehensive study expands our understanding of the genus Nakazawaea and highlights the ecological and industrial potential of these yeasts in fermentation processes. The holotype of N. atacamensis sp. nov. is CBS 18375 T . The Mycobank number is MB 849680.

DOI: 10.22541/au.169663687.70782696/v1

Authors: Francisco Cubillos, Macarena Araya, Tomás Moyano, Pablo Villarreal, Ana Raquel Santos, Francisca P Diaz, Andrea Bustos-Jarufe, Kamila Urbina, Marizeth Goenewald, Rodrigo A. Gutiérrez, Carlos Rosa

Institutions: Universidad de Santiago de Chile, Millennium Institute for Integrative Biology, Universidade Federal de Minas Gerais, Pontificia Universidad Católica de Chile, Westerdijk Fungal Biodiversity Institute, Millennium Institute, Institute of Ecology and Biodiversity

Language: English

Topic: Yeasts and Rust Fungi Studies

Subfield: Microbiology

Field: Immunology and Microbiology

Domain: Life Sciences

Open Access status: green

Funders: Universidad de Santiago de Chile, Institut Català de Nanociència i Nanotecnologia, Conselho Nacional de Desenvolvimento Científico e Tecnológico, Fundação de Amparo à Pesquisa do Estado de Minas Gerais, Agencia Nacional de Investigación y Desarrollo

Awards: CARACTERIZAÇÃO DA PRODUÇÃO DE BIOETANOL E XILITOL POR ISOLADOS DA LEVEDURA CANDIDA MATERIAE, G3790935329, G4529709011, SELEÇÃO DE LINHAGENS DE SACCHAROMYCES CEREVISIAE PARA A PRODUÇÃO NACIONAL DE CERVEJAS ARTESANAIS, IDENTIFICACION DE REGULADORES DE RESPUESTA A SEQUIA MEDIANTE REDES REGULATORIAS CONSERVADAS EN PLANTAS, GENOMIC AND TRANSCRIPTIONAL PROFILING OF FERMENTATIVE VIGOR IN LABORATORY LAGER YEAST HYBRIDS., G5284675477, G5473879402, G573291573, G5905040377, BIODIVERSIDADE DE LEVEDURAS BRASILEIRAS APLICADA À PRODUÇÃO DE ETANOL DE SEGUNDA GERAÇÃO, G741624880

OpenAlex ID: W4387394591

Cites: 48

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Related to: 10