Year: 2023
Type: article
Abstract: In recent years, the emergence of serial crystallography, initially pioneered at X-ray free-electron lasers (XFELs), has sparked a growing interest in collecting macromolecular crystallographic data at room temperature. Various fixed-target serial crystallography techniques have been developed, ranging from commercially available chips to in-house designs implemented at different synchrotron facilities. Nevertheless, there is currently no commercially available chip (known to the authors) specifically designed for the direct handling of oxygen-sensitive samples. This study presents a methodology employing silicon nitride chips arranged in a `sandwich' configuration, enabling reliable room-temperature data collection from oxygen-sensitive samples. The method involves the utilization of a custom-made 3D-printed assembling tool and a MX sample holder. To validate the effectiveness of the proposed method, deoxyhemoglobin and methemoglobin samples were investigated using the BioMAX X-ray macromolecular crystallography beamline, the Balder X-ray absorption spectroscopy beamline and UV-Vis absorption spectroscopy.
Institutions: Lund University, MAX IV Laboratory
Language: English
Subfield: Structural Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,740
Awards: G1849099000, G208125138, G317939215, G3728818118, G5096929151, MAX IV operations – Formas, G5224014036, G5229471624, G594270511, G712881263, G7769948153, MAX IV operations 2019-2021, Expanding the domain of protein serial crystallography: membrane protein & in cellulo crystallization, G8674945231, G8693631393
OpenAlex ID: W4387798529