Year: 2023
Type: preprint
Abstract: Secretory proteins are sorted at the trans-Golgi network (TGN) for export into specific transport carriers. However, the molecular players involved in this fundamental process remain largely elusive. Here, we identified the transmembrane protein TGN46 as a receptor for the export of secretory cargo protein PAUF in CARTS – a class of protein kinase D-dependent TGN-to-plasma membrane carriers. We show that TGN46 is necessary for cargo sorting and loading into nascent carriers at the TGN. By combining quantitative fluorescence microscopy and mutagenesis approaches, we further discovered that the lumenal domain of TGN46 encodes for its cargo sorting function. In summary, our results define a cellular function of TGN46 in sorting secretory proteins for export from the TGN.
Source: eLife
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,500
Funders: Institute for Research in Biomedicine, Fundación Cellex, Centres de Recerca de Catalunya, Laserlab-Europe, Institut de Ciències Fotòniques, Universitat Pompeu Fabra, Generalitat de Catalunya, Ministerio de Ciencia e Innovación, FUNDACIÓ Privada MIR-PUIG, European Research Council, Agència de Gestió d'Ajuts Universitaris i de Recerca, Agencia Estatal de Investigación
OpenAlex ID: W4387908930