Quercetin Change the Exosome Secretion and Total miRNA Concentration in Primary (Colo320) and Metastatic (Colo741) Colon Cancer Cell Lines
Year: 2023
Type: article
Abstract: Objective: Quercetin, which is considered a potential anti-cancer agent in the prevention of colon cancer, is one of its natural polyphenolic compounds.Extracellular vesicles, such as exosomes, secreted from cells and their components contribute to cellular behavioral characteristics by transporting proteins or miRNAs.In this study, we aimed to determine the cytotoxicity of Dicer, Ago2, eIF2α CD9 and CD63 and their effects on exosomal miRNA secretion and expression in Colo320 and Colo741 colon cancer cell lines applied quercetin. Methods:The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to analyze the cytotoxicity of quercetin.MTT analysis is a colorimetric analysis method applied to measure the metabolic activity of cells.The absorbance was measured at 570 nm by a spectrophotometer.Besides that, the indirect immunoperoxidase staining was used for the distribution of Dicer, Ago2, eIF2α, CD9, and CD63 in Colo320 and Colo741.Total miRNA in exosome was determined with miRCURY™ Kit.Results: The immunoreactivities of eIF2α and CD9 significantly differed compared to the Colo741 control group after quercetin application.In addition, exosomal miRNA concentrations were higher in both quercetin applied-Colo320 and Colo741 cells.Amaç: Kolon kanserinin önlenmesinde potansiyel bir anti-kanser ajan olarak kabul edilen kersetin, doğal polifenolik bileşiklerinden biridir.Hücrelerden ve bileşenlerinden salgılanan eksozom gibi hücre dışı veziküller, proteinleri veya miRNA'ları taşıma yoluyla hücresel davranış özelliklerine katkıda bulunur.Bu çalışmada, kersetin uygulanan Colo320 ve Colo741 kolon kanseri hücre dizilerinde Dicer, Ago2, eIF2α CD9 ve CD63'ün sitotoksisitesini ve eksozomal miRNA salgılanması ve ekspresyonu üzerine etkilerini belirlemeyi amaçladık.Yöntemler: Kersetin sitotoksisitesinin analizi için MTT testi kullanıldı.Colo320 ve Colo741'de Dicer, Ago2, eIF2a, CD9 ve CD63'ün dağıtımı için dolaylı immünoperoksidaz boyaması kullanıldı.Eksozomdaki toplam miRNA, miRCURY™ Kit ile belirlendi.Bulgular: Kersetin uygulamasından sonra eIF2a ve CD9 immünoreaktiviteleri Colo741 kontrol grubuna kıyasla önemli ölçüde farklılık gösterdi.Ayrıca eksozomal miRNA konsantrasyonları hem kersetin uygulanan Colo320 hem de Colo741 hücrelerinde daha yüksekti.
Source: Bezmialem Science
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4387939770