Work

Mind your tag in single-molecule measurements

Year: 2023

Type: article

Abstract: In this issue of Cell Reports Methods, Molina and colleagues use in vitro single-molecule DNA flow-stretching to demonstrate the severe effects of appending a short lysine-cysteine-lysine (KCK) tag on the Bacillus subtilis ParB protein. This assay could be further utilized to evaluate the impact of other tags on DNA-binding proteins.

Source: Cell Reports Methods

DOI: 10.1016/j.crmeth.2023.100623

Authors: Raquel Merino Urteaga, Taekjip Ha

Institutions: Boston Children's Hospital, Johns Hopkins University, Howard Hughes Medical Institute, Harvard University

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $5,200

OpenAlex ID: W4387966913

FWCI: 0.436

Cites: 12

Cited by: 4

Related to: 10