Viral vector-mediated transgene delivery with novel recombinase systems for targeting neuronal populations defined by multiple features
Year: 2023
Type: article
Abstract: A comprehensive understanding of neuronal diversity and connectivity is essential for understanding the anatomical and cellular mechanisms that underlie functional contributions. With the advent of single-cell analysis, growing information regarding molecular profiles leads to the identification of more heterogeneous cell types. Therefore, the need for additional orthogonal recombinase systems is increasingly apparent, as heterogeneous tissues can be further partitioned into increasing numbers of specific cell types defined by multiple features. Critically, new recombinase systems should work together with pre-existing systems without cross-reactivity in vivo. Here, we introduce novel site-specific recombinase systems based on ΦC31 bacteriophage recombinase for labeling multiple cell types simultaneously and a novel viral strategy for versatile and robust intersectional expression of any transgene. Together, our system will help researchers specifically target different cell types with multiple features in the same animal.
Source: Neuron
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $9,080
Funders: National Science Foundation, National Research Foundation, National Research Foundation Singapore, National Research Foundation of Korea, National Institutes of Health, National Institute of Mental Health, National Institute on Drug Abuse, National Institute of Neurological Disorders and Stroke
Awards: G2486395883, Neural Circuits Postdoctoral Training Program, G323301830, G4137185153, Anatomical characterization of neuronal cell types of the mouse brain, EAGER: Development of the Novel Recombinase System for the Intersectional Dissection of Neural Circuitry, G8043279009, Role of Glutamatergic Neurons in External Globus Pallidus in the Behavioral Deficits in Animal Models of Progressive Dopamine Depletion, Dissecting the role of ventral pallidal circuitry in cocaine seeking after the withdrawal
OpenAlex ID: W4388024587