Work

A Simple and Efficient UPLC-ESI-Q-TOF-MS Method for Determining the Chemical Composition in Rhizomes of Multiple Stems Paris polyphylla var . yunnanensis

Year: 2023

Type: article

Abstract: Due to the clinical significance of steroidal saponins, multiple stems Paris polyphylla var. yunnanensis (MPPY) has attracted much interest and played a crucial role in drug development. However, developing a simple and efficient method to analyze the chemical composition of MPPY remains a pronounced challenge. Herein, we report for the first time the component analysis of MPPY rhizomes using the chromatographic fingerprint combined with ultra-high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-ESI-Q-TOF-MS) method. Nineteen compositions of MPPY were qualitatively investigated by such strategy and confirmed as steroidal saponins. Among these compositions, 17 steroidal saponins were detected in 10 different batches of MPPY. Thirteen common peaks appeared in chromatographic fingerprint, of which 11 common peaks can be attributed by LC–MS data. The experimental facts demonstrate that the method established is simple, rapid, and reliable to evaluate the quality of MPPY. Besides, the correlation and difference of steroidal saponins in the rhizomes of MPPY from different places were also studied.

Source: Natural Product Communications

DOI: 10.1177/1934578x231207524

Authors: Xue He, Lin Jin, Qicui Wang, Anzhong Peng, Haifeng Li

Institution: Dali University

Language: English

Topic: Traditional Chinese Medicine Analysis

Subfield: Complementary and alternative medicine

Field: Medicine

Domain: Health Sciences

Open Access status: gold

Estimated APC paid: $2,000

Funder: National Natural Science Foundation of China