FIGURE 2 from PSGL-1 Blockade Induces Classical Activation of Human Tumor-associated Macrophages
Year: 2023
Type: other
Abstract: PSGL-1 is expressed on human M2-like macrophages. A, Schematic of M1, M0, and M2c macrophage differentiation and polarization conditions using human peripheral blood monocytes. B, PSGL-1 expression on M0, M2c, and M1-like macrophages. Data are presented as an average MFI across 4 donors and is representative of six experiments. C, siRNA knockdown of PSGL-1 in M2c macrophages transfected with 25 nmol/L PSGL-1 siRNA LNPs on days 1 and 3 during the differentiation and polarization period. PSGL-1 mRNA and protein levels were measured on day 8 by branched chain DNA analysis and flow cytometry, respectively (n = 4 donors). D, Fold change of M2c markers CD16, CD163, and CD206 as determined by flow cytometry in M2c macrophages treated with siRNA LNPs relative to those treated with Luciferase siRNA. Significance measured using Student t test in Graph Prism software for each pair of phenotypic macrophage conditions (B) or siRNA knockdown and phenotypic change (C, D).
Authors: Kevin Kauffman, Denise Manfra, Dominika Nowakowska, Mohammad Zafari, Phuong A. Nguyen, Ryan Phennicie, Elisabeth H. Vollmann, Brian O'Nuallain, Sara Basinski, Veronica Komoroski, Kate Rooney, Elizabeth K. Culyba, Joseph Wahle, Carola Ries, Michael Brehm, Steve Sazinsky, Igor Feldman, Tatiana I. Novobrantseva
Language: English
Topic: Immune cells in cancer
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4388199032