Year: 2023
Type: conference-paper
Abstract: Media for acclimatization is one of the important factors for the success of plant propagation through tissue culture techniques, because the regenerated plantlets do not yet have a well-developed root system.This situation causes plantlets to be very sensitive to environmental stresses, both abiotic and biotic.Therefore, plantlet acclimatization requires special handling, and modifications to environmental conditions are needed, especially in relation to growing media.This study aimed to obtain the best media composition and dose of arbuscular mycorrhizal fungi (AMF) to support the growth of banana plantlets during the acclimatization stage.This investigation was conducted at the Plant Biotechnology Laboratory Faculty of Agriculture University of Jambi for approximately 4 months.Tissue culture generated plantlets of banana cv.Barangan were acclimatisized on media consisted of s-oil and trichocompost (1:1, 1:2 and 2:1) and AMF (0, 10, and 20 g per plantlet).The trial was arranged in a factorial randomized block design with 3 replications, making the total of 27 experimental units.Each unit consisted of 10 polybags containing one individual plant.Data were recorded on the percentage of live seedlings (survival rate), increase in plant height, increase in number of leaves, increase in stem diameter, length, and width of fully developed leaves.Statistical analysis using Analysis of Variance, followed by the Least Significant Difference test at 5% confidence interval was employed to see the effect of the media compositions and AMF on plantlet growth.Results showed that all transplanted plantlets suceeded acclimatization process (100% survival rate).Statistical analysis revealed that either the combination of media compositions and dosages of AMF or AMF alone did not show any significant effect on all variables observed.The media composition, however, was found to have significant effect on plant height, leaf number, and leaf length, but not on stem diameter and leaf width.This investigation confirmed benefit of the use of trichocompost in acclimatization media, in which the composition of 2:1 of soil and trichocompost gave the best the growth of banana plantlets during acclimatization.
Institution: Universitas Jambi
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W4388308348