Non-additive genetic components contribute significantly to population-wide gene expression variation
Year: 2023
Type: article
Abstract: Gene expression variation, an essential step between genotype and phenotype, is collectively controlled by local (cis) and distant (trans) regulatory changes. Nevertheless, how these regulatory elements differentially influence gene expression variation remains unclear. Here, we bridge this gap by analyzing the transcriptomes of a large diallel panel consisting of 323 unique hybrids originating from genetically divergent Saccharomyces cerevisiae isolates. Our analysis across 5,087 transcript abundance traits showed that non-additive components account for 36% of the gene expression variance on average. By comparing allele-specific read counts in parent-hybrid trios, we found that trans-regulatory changes underlie the majority of gene expression variation in the population. Remarkably, most cis-regulatory variations are also exaggerated or attenuated by additional trans effects. Overall, we showed that the transcriptome is globally buffered at the genetic level mainly due to trans-regulatory variation in the population.
Source: Cell Genomics
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $8,900
Funders: Agence Nationale de la Recherche, Institut National de la Santé et de la Recherche Médicale, Université de Strasbourg, Centre National de la Recherche Scientifique, Institut Universitaire de France, National Institutes of Health, European Research Council, National Institute of General Medical Sciences
Awards: Par-delà les frontières, l'Université de Strasbourg, G2255650664, G2694206344, G2716474586, G3357951378, G471331628, G7240651115, Dissecting the impact of genetic backgrounds on the phenotypic landscape in natural population, Species-wide survey of the phenotypic impact of genomic structural variation in yeast
OpenAlex ID: W4389305331