Antiproliferative, apoptosis-inducing, and GSTP1 demethylation activities of Ellagitannins isolated from Cornus alba L.
Year: 2023
Type: article
Abstract: This study aimed to prove the prostate cancer chemopreventive activity of compounds isolated from CA. We evaluated these compounds using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and evaluated their NF-κB inhibitory activity and apoptosis-inducing activity using western blot analysis and flow cytometry, respectively. Their DNA methylation activity was also evaluated via a methylation-specific polymerase chain reaction in androgen-dependent (LNCaP) and androgen-independent (PC-3) prostate cancer cell lines. Camptothin B (1), cornusiin B (2), and cornusiin A (3), which were isolated in our previous work, relatively reduced the protein expression levels in PCa cells. Among them, cornusiin B (2) exhibited excellent NF-κB inhibitory activity. Also, concentration-dependently increased the unmethylated DNA content and decreased the methylated DNA content in both PC-3 and LNCaP cells. Therefore, cornusiin B (2), which was isolated from CA, has the potential to act as a chemopreventive agent for prostate cancer.
Source: Natural Product Research
Authors: Siyeon Jin, Seoyeon Seonu, Jun Yin, Seyeon Son, Jinhyeok Choi, Jaeyoon Oh, Kyungmi Kim, Minwon Lee
Institutions: Chung-Ang University, Novarex (South Korea)
Language: English
Subfield: Pharmacology
Domain: Life Sciences
Open Access status: green
Funder: Korea Forest Service
Award: G972932952
OpenAlex ID: W4390144522