Isolation, Characterisation and In-vitro Antioxidant activities of Flavonoid Compounds from Methanolic fraction of Aspidopterys indica
Year: 2023
Type: article
Abstract: Aspidopterys indica belongs to the family Malphigiaceae and is used to treat hypertension and skin diseases. In the current investigation, methanolic extract was fractionated using vacuum liquid chromatography, and the methanolic fraction was subjected to column chromatography for isolation and characterized by fourier transform infrared spectroscopy (FTIR), Mass, proton nuclear magnetic resonance (1H-NMR), and carbon-13 nuclear magnetic resonance (13C-NMR) spectroscopy. The compounds isolated were assessed for their in-vitro DPPH antioxidant activity. Two compounds, AI-1 and AI-2, established after spectral analysis, were identified as catechin flavan-3-ol and isoorientin flavone C-glucoside. The results indicated that the compounds showed concentration-dependant DPPH scavenging action and IC50 of catechin 93.66 μg/mL, isoorientin 92.09 μg/mL. This study concludes that catechin and isoorientin with known antioxidant potential were isolated from the methanolic fraction of A. indica, which could effectively manage oxidative stress-related diseases.
Authors: Udaya C P, Sunitha K
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W4390888231