Heteroatom-tagged proteomics of lung cancer and chronic obstructive pulmonary disease human serum reveal alterations in selenoproteins
Year: 2024
Type: article
Study design: Observational Study
Abstract: Heteroatom-tagged proteomics allows the absolute quantification of selenoproteins using the heteroatom as a “tag” into a selective and sensitive atomic detector instead of a molecular one. Using this analytical method, about 90% of total selenium in human serum/plasma can be measured as selenoproteins and total selenometabolites and thus, the status of selenium can be determined. Herein, we determined the absolute concentration of selenoproteins in human serum patients with lung cancer (LC) and chronic obstructive pulmonary disease (COPD), a competing cause of morbidity and mortality in smokers as well as an independent risk factor for LC. We conducted an observational study of 154 human serum samples obtained from LC and COPD patients with varying severity of disease, including COPD patients who developed LC during follow-up and healthy controls (HC). Using heteroatom-tagged proteomics, we determined extracellular glutathione peroxidase (eGPx), selenoprotein P (SELENOP), and selenoalbumin (SeAlb). Associations between selenoproteins were also studied as potential biomarkers of disease. The concentration of eGPx was significantly higher in the all-inclusive COPD cohort compared to HC, COPD patients with LC, or those with mild obstructive lung disease, while SELENOP concentration was significantly decreased in LC patients compared to HC and COPD. We found an inverse correlation between SELENOP and SeAlb in HC, but also in LC patients, and especially in patients with COPD and LC. Moreover, we found that eGPx and selenometabolite concentrations were positively associated with LC human serum. Selenoprotein concentrations were altered in COPD and LC when compared to healthy controls suggesting a potential role of the selenoproteome in the diagnosis and/or treatment of these tobacco-related diseases.
Source: Microchemical Journal
Authors: Belén Callejón-Leblic, Saida Sánchez Espirilla, Carolina Gotera-Rivera, Rafael Santana, Isabel Díaz-Olivares, José María Marín Trigo, Ciro Casanova Macario, Borja G. Cosio, Antonia Fuster, Ingrid Solanes García, Juan P. de-Torres, Nuria Feu Collado, Carlos Cabrera Lopez, Carlos Amado Diago, Amparo Romero Plaza, Luis Alejandro Padrón Fraysse, Eduardo Márquez Martín, Margarit Marín Royo, Eva Balcells Vilarnau, Antonia Llunell Casanovas, Cristina Martínez González, Juan Bautista Galdíz Iturri, Celia Lacárcel Bautista, José Luis Gómez-Ariza, Antonio Pereira-Vega, Luis Seijo, José Luis López-Campos, Germán Peces-Barba, Tamara García-Barrera
Institutions: Universidad de Huelva, National University of Saint Anthony the Abbot in Cuzco, Instituto de Salud Carlos III, Fundación Andaluza Beturia para la Investigación en Salud, Hospital Universitario Miguel Servet, Universidad de La Laguna, Hospital Universitario Nuestra Señora de Candelaria, Centro de Investigación Biomédica en Red de Enfermedades Respiratorias, Health Research Institute of the Balearic Islands, Hospital Universitario Son Espases, Hospital Son Llatzer, Hospital de Sant Pau, Clinica Universidad de Navarra, Instituto Maimónides de Investigación Biomédica de Córdoba, Hospital Universitario Reina Sofía, Hospital Universitario de Gran Canaria Doctor Negrín, Marqués de Valdecilla University Hospital, Fundación Hospital Manacor, Hospital Juan Ramón Jiménez, Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío, Hospital Del Mar, Hospital Terrassa, Hospital Universitario Central de Asturias, Hospital de Cruces, Complejo Hospitalario de Jaén, Centro de Investigación Biomédica en Red, Universidad de Sevilla
Language: English
Subfield: Nutrition and Dietetics
Field: Nursing
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $3,720
Awards: G434091683, G5172722842, G5348269913
OpenAlex ID: W4391132207