Impact of Liposomes on the Effects of Quercetin-Rich Flavonoid Fraction Extracted from French Marigold (Tagetes patula L.) on the Jurkat Cells
Year: 2024
Type: article
Abstract: Background: Natural polyphenols are widely used in traditional medicine for treating various diseases.Despite healthful properties, phenolic compounds ingestion in the form of food does not provide concentration (due to their low solubility in water, poor absorption, and fast metabolism) enough to achieve systemic therapeutic effects in the body.This problem has been solved by creating various composite pharmaceuticals from phenolic compounds using different methods to ensure the stabilization of polyphenols.In the present study, we investigated the effects of quercetin-rich flavonoid fraction extracted from French Marigold (Tagetes patula L.) alone and incorporated into DPPA (1,2-palmitoyl phosphatidic acid) and DPPC (dipalmitoyl phosphatidylcholine) liposomes on the viability of the intact and incubated under oxidative stress conditions Jurkat cells. Materials and Methods:Quercetin-rich flavonoid fraction was extracted from a French Marigold (Tagetes patula L.) by TLC, HPLC, and LC-MS methods.Extract alone and in complex with Dipalmitoyl phosphatidylcholine (DPPC) and 1,2-dipalmitoyl phosphatidic acid (DPPA) liposomes were added to the Jurkat cells culture at a rate of 2 mg/mL -1 .Cell viability was assayed by the MTT test based on evaluating cellular dehydrogenase activity.Results: The spectrophotometric absorption spectrum of the quercetin-rich flavonoid fraction extracted from French Marigold (Tagetes patula L.) shows two peaks corresponding to benzoyl (254nm) and cinnamyl (375nm) aromatic rings.In the complex of quercetin-rich flavonoid fraction with DPPC and DPPA liposomes, the spectrophotometric absorption peak at 254nm was not detected, while the absorption intensity of the peak at 375nm was sharply reduced.The quercetin-rich flavonoid fraction alone and in combination with DPPC liposome increased intact and incubated under low-and high-intensity oxidative stress conditions Jurkat cells' viability but did not revealed effect in combination with DPPA liposome. Conclusion:The quercetin-rich flavonoid fraction extracted from French Marigold (Tagetes patula L.) formts stable complexes with DPPC and DPPA liposomes that gives possibility to storage of high content of phenolic compounds in lipid nanocapsules.Negative (-P-COO-) surface charge of DPPA liposomes tends their repelling by negatively charged cellular membranes, whereas polar phosphatidylcholine head of DPPC liposomes which contains as negative (-P-COO-) also the positive (N + ) charged groups is attracted to the cellular membrane, leads the release of quercetin-rich flavonoid fraction extracted from French Marigold (Tagetes patula L.) within the cell cytoplasm and ensures an enhancement efficiency of the delivery process.
Source: Acta Scientific Medical Sciences
Institution: Tbilisi State Medical University
Language: English
Subfield: Biochemistry (medical)
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4391749415