Work

Correction: LncRNA SNHG1 and RNA binding protein hnRNPL form a complex and coregulate CDH1 to boost the growth and metastasis of prostate cancer

Year: 2024

Type: erratum

Abstract: A Hierarchical clustering of 1045 transcripts altered (≥1.5-fold change) in NC-treated cells and shRNA SNHG1-treated cells with three repeats. B , C KEGG analysis and differential genes GSEA enrichment analysis demonstrated that cell adhesion molecules are the potential targets of SNHG1 pathway. D Volcanic map analysis of differential genes. E The altered mRNA levels of genes were selectively confirmed by qRT-PCR in knockdown SNHG1. F SDS-PAGE silver staining of RNA pull-down protein samples showed a significant difference in protein band from 55 to 70 kDa. G Differential protein band mass spectrometry showed that the protein was hnRNPL. H The interaction possibilities of hnRNPL and SNHG1 were detected in RPIseq, and the results showed that hnRNPL could well bind with SNHG1 well (RPISeq). I Pull-down assays showed that biotinylated SNHG1 could retrieve hnRNPL in DU145 cells by western blot(flow-through was a input control). J RNA immunoprecipitation revealed that hnRNPL could also specifically bind to SNHG1. K , L The western blotting results after SNHG1 knockdown in DU145 and C4-2 cells showed that the expression level of hnRNPL did not change, while E-cad and Claudin-1 were significantly up-regulated, Vimentin, Slug and ZEB1 were significantly decreased. overexpression of SNHG1 showed an opposite effect of these protein in in DU145 and C4-2 cells. Error bars indicate means ± SD. *** P < 0.001, **** P < 0.0001.

Source: Cell Death and Disease

DOI: 10.1038/s41419-024-06561-5