Work

Patchy and widespread distribution of bacterial translation arrest peptides associated with the protein localization machinery

Year: 2024

Type: article

Abstract: Regulatory arrest peptides interact with specific residues on bacterial ribosomes and arrest their own translation. Here, we analyse over 30,000 bacterial genome sequences to identify additional Sec/YidC-related arrest peptides, followed by in vivo and in vitro analyses. We find that Sec/YidC-related arrest peptides show patchy, but widespread, phylogenetic distribution throughout the bacterial domain. Several of the identified peptides contain distinct conserved sequences near the C-termini, but are still able to efficiently stall bacterial ribosomes in vitro and in vivo. In addition, we identify many arrest peptides that share an R-A-P-P-like sequence, suggesting that this sequence might serve as a common evolutionary seed to overcome ribosomal structural differences across species.

Source: Nature Communications

DOI: 10.1038/s41467-024-46993-3

Authors: Keigo Fujiwara, Naoko Tsuji, Mayu Yoshida, Hiraku Takada, Shinobu Chiba

Institution: Kyoto Sangyo University

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $6,790

Funders: Institute for Fermentation, Osaka, Ministry of Education, Culture, Sports, Science and Technology, Japan Society for the Promotion of Science