Work

Spatial Mapping and Host Linking of Mobile Genetic Elements in Complex Microbiomes - Combined taxonomic mapping and MGE mapping

Year: 2023

Type: dataset

Abstract: We used rRNA FISH to stain five common oral genera, Veillonella, Streptococcus, Corynebacterium, Lautropia, and Neisseria, each with a different fluorophore, and we used MGE-FISH to stain the termL gene of an active prophage with a sixth fluorophore. We chose a target panel of 18 genera that are highly abundant and prevalent in human plaque and designed a HiPR-FISH probe panel using a 5-fluorophore combinatorial barcoding scheme. Using MGE-FISH, we stained a plasmid carrying mefE, subunit of a major-facilitator-superfamily antibiotic efflux pump.

Source: Zenodo (CERN European Organization for Nuclear Research)

DOI: 10.5281/zenodo.8016062

Authors: Grodner, Benjamin, Shi, Hao, Farchione, Owen, Vill, Albert C, Ntekas, Ioannis, Diebold, Peter J, Zipfel, Warren R, Brito, Ilana L, De Vlaminck, Iwijn

Institution: Cornell University

Language: English

Topic: Oral microbiology and periodontitis research

Subfield: Periodontics

Field: Dentistry

Domain: Health Sciences

Open Access status: green

OpenAlex ID: W4393751840

Cites:

Cited by:

Related to: 10