Year: 2024
Type: article
Abstract: Circadian clocks temporally coordinate daily organismal biology over the 24-h cycle. Their molecular design, preserved between fungi and animals, is based on a core-oscillator composed of a one-step transcriptional-translational-negative-feedback-loop (TTFL). To test whether this evolutionarily conserved TTFL architecture is the only plausible way for achieving a functional circadian clock, we adopted a transcriptional rewiring approach, artificially co-opting regulators of the circadian output pathways into the core-oscillator. Herein we describe one of these semi-synthetic clocks which maintains all basic circadian features but, notably, it also exhibits new attributes such as a "lights-on timer" logic, where clock phase is fixed at the end of the night. Our findings indicate that fundamental circadian properties such as period, phase and temperature compensation are differentially regulated by transcriptional and posttranslational aspects of the clockworks.
Source: The EMBO Journal
Language: English
Subfield: Endocrine and Autonomic Systems
Field: Neuroscience
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $6,890
Awards: G2218878644, G2702155792, Identification and Analysis of Circadian Clock-Controlled Genes, G4634471397, G480629005, MODULAR REDESIGN AND REWIRING OF CIRCADIAN CIRCUITS TO CHALLENGE DESIGN PRINCIPLES AND DISSECT FUNDAMENTAL CLOCK PROPERTIES, G5284675477, MAPEO DE LA RED TRANSCRIPCIONAL ASOCIADA A LA DEGRADACION DE CELULOSA EN NEUROSPORA CRASSA PARA LA GENERACION DE CEPAS CON POTENCIAL BIOTECNOLOGICO, Genetic and Molecular Dissection of the Neurospora Clock
OpenAlex ID: W4394844752