OsACL-A2 is involved in iron transport and positively regulates iron dependent disease resistance
Year: 2024
Type: preprint
Abstract: Iron is an essential nutrient required for growth, development and also impacts disease resistance in plants. Plant absorb iron through their roots and citrate plays a vital role in transport of Fe 3+ through the xylem. In this study, we found that the rice cytoplasmic ATP-citrate lyase subunit A2, OsACL-A2 ( Os12g0566300 ) serves as a crucial factor in iron uptake and translocation. Leaf iron levels were depressed in the osacl-a2 mutant which caused iron deficiency induced chlorosis, activated defense signaling and eventually necrosis in mature leaves. Furthermore, loss of OsACL-A2 activity reduced disease resistance in leaves that had not reached development of necrosis. Citrate levels are increased in the osacl-a2 mutant, while ACL activity in the wild type is upregulated in response to both iron starvation and pathogen attack. We hypothesize that OsACL-A2 activity generates source-sink gradients through targeted lysis activity thus directing the movement of citrate-Fe 3+ .
Authors: Wenjing Duan, Aaron Chan, Peng Xu, Yingxin Zhang, Lianping Sun, Beifang Wang, Yongrun Cao, Yue Zhang, Min Jiang, Dian Li, Daibo Chen, Yongbo Hong, Xiaodeng Zhan, weixun wu, Shihua Cheng, Qunen Liu, Liyong Cao
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
OpenAlex ID: W4395089340